Saikosaponin D inhibits epithelial mesenchymal transition of
A549 cells via mTOR signaling pathway
SHI Xiao-dong1, ZHENG Jin-xu1, QIAN Hai2, YANG Lei1, SUN Jin-ling1
（1. Department of Respiratory Medicine, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001;2.School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013, China )
（1. Department of Respiratory Medicine, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001;2.School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013, China )
Abstract：Objective: To study the effect and mechanism of saikosaponin D (SSd) on transforming growth factorβ1(TGFβ1) induced epithelial mesenchymal transition (EMT) in A549 cells. Methods: A549 cells were cultured in vitro, and EMT was induced by TGFβ1 (10 ng/mL). The inhibitory effects of different concentrations of SSd( 0.5, 1.0, 2.0 μmol/L) were observed. Cell proliferation activity was analyzed by MTT assay, protein expression levels of mTOR, p70S6, αsmooth muscle actin (αSMA), Ncadherin, type Ⅰ collagen(collagen Ⅰ) and Ecadherin were detected by Western blotting. Results: The proliferation of A549 cells stimulated by TGFβ1 was enhanced, and SSd significantly reversed the proliferation of TGFβ1stimulated cells in a dosedependent manner. After treatment with SSd, the expression of mTOR and downstream p70S6 protein were significantly decreased in TGFβ1 stimulated A549 cells, and the expression levels of αSMA, collagen Ⅰ and Ncadherin were significantly decreased, while Ecadherin was significantly increased in the dose and timedependent manner. Conclusion: SSd can inhibit A549 cells EMT via a mTOR signaling pathway.
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2019, Vol. 29 
