（1. Zhenjiang Food and Drug Supervision and Inspection Center, Zhenjiang Jiangsu 212002; 2. School of Pharmacy, Jiangsu University, Zhenjiang Jiangsu 212013, China)
Abstract：Objective: To determine the decoction and drug-containing serum of Sijunzi decoction by high performance liquid chromatographymass spectrometry(HPLC-MS), and analyse the major metabolites of Sijunzi decoction. Methods: The decoction was prepared according to the proportion of traditional Chinese medicinal materials of sijunzi decoction, and the blood was collected 2 h after intragastric administration Sijunzi decoction to 6 rats. HPLC-MS was used to identify the active components in decoction and drug serum. Results: According to the total ion chromatogram obtained by HPLC-MS and the fragmentation peak of mass spectrometry, it was deduced that Sijunzi decoction contained 10 chemical substances, among them, 7 saponins were ginsenoside Rb1, Re, Rg1, Rb2, Rd, Rg3, glycyrrhizic acid; 3 flavonoids were liquiritin, glycyrrhizin, licorice coumarin. Drug-containing serum contains three prototype chemical composition, which are ginsenoside Rb1, Re, glycyrrhizic acid and 2 transitional components, glycyrrhetinic acid and glycyrrhizin glucuronide. Conclusion: The chief chemical compositions of the decoction and drug-containing serum of Sijunzi decoction were ginsenosides and glycyrrhiza, the former was metabolized in blood by prototype compound while the latter was metabolized in blood by hydrolysis product.
收稿日期: 2018-09-13
基金资助:
镇江市科技计划资助项目(SH2016020); 江苏大学第十七批大学生科研项目
通讯作者:
徐卫东(通讯作者)，博士，副教授，E-mail：xuwd@ujs.edu.cn
作者简介: 尚如霞(1978—)，女，江苏镇江人，副主任药师，硕士，主要从事药品质量分析与研究；
引用本文:
尚如霞1， 徐卫东2， 许晓倩2， 彭云2. 四君子汤血清药效成分的HPLC-MS分析[J]. 江苏大学学报:医学版, 2018, 28(06): 529-.
SHANG Ru-xia1, XU Wei-dong2, XU Xiao-qian2, PENG Yun2.
Analysis of the major metabolites of Sijunzi decoction in rat serum based on HPLC-MS. Journal of Jiangsu University(Medicine Edition), 2018, 28(06): 529-.
2018, Vol. 28 
