miR-187在胃癌中的表达及其对胃癌细胞增殖和侵袭的影响

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摘要目的: 探讨microRNA187(miR187)在胃癌中的表达及其对胃癌增殖、侵袭和迁移的影响。方法: 收集24对人胃癌组织和癌旁正常组织，采用实时定量PCR检测miR187的表达，蛋白质印迹法检测CD276的表达，分析miR187与CD276表达的相关性，并分析这两项指标与各临床指标的关系。用转染沉默法分别抑制人胃腺癌细胞SGC7901中miR187和CD276的表达；用CCK8法检测SGC7901细胞增殖率；用Transwell法检测SGC7901细胞侵袭性及迁移性；蛋白质印迹法检测SGC7901细胞中MMP9的表达。结果：与癌旁组织比较，miR187在胃癌肿瘤组织中表达明显升高(P＜0.01)，在Ⅰ期、Ⅱ期、Ⅲ期和Ⅳ期胃癌组织表达呈进行性升高趋势；胃癌组织CD276表达均明显降低(P＜0.01)；胃癌组织中miR187表达与CD276表达呈负相关，决定系数R2值为0.840；miR187在SGC7901表达高于人胃黏膜GES细胞(P＜0.01)。与对照组比较，miR187 抑制剂能明显抑制SGC增殖，CD276siRNA促进SGC增殖(P＜0.01)；miR187 抑制剂能明显抑制SGC侵袭和迁移，CD276siRNA促进SGC7901细胞的侵袭和迁移。蛋白质印迹检测结果显示，miR187抑制剂组CD276表达明显升高(P＜0.05)，MMP9表达显著降低(P＜0.05)；CD276siRNA组MMP9表达明显升高(P＜0.05)。结论： miR187可能是通过下游基因CD276，参与胃癌的增殖及迁移。

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关键词 ： miR-187, 胃癌, 增殖, 侵袭, 移

Abstract：jective: To investigate the effect of abnormal expression of microRNA187(miR187) on proliferation, invasion and migratory of gastric cancer. Methods: A total of 24 pairs of human gastric cancer tumor and matched nontumor tissues were collected and miR187 expression was detected and their relevance was analyzed. The expression of miR187 and CD276 in human gastric cancer cell SGC7901 were inhibited by siRNA transfection, and then the effect of SGC7901 cell proliferation and invasionmigration was detected by CCK8 and Transwell assay respectively. The expression of matrix metalloproteinase9(MMP9) was detected by Western blotting. Results: Compared with matched nontumor tissues, the expression of miR187 was increased in stage Ⅰ,Ⅱ,Ⅲ and Ⅳ gastric cancer(P＜0.01); the expression of CD276 was decreased in stage Ⅰ,Ⅱ,Ⅲ and Ⅳgastric cancer(P＜0.01). The correlation coefficient of miR187 expression and CD276 expression in gastric carcinoma was 0.840. Compared with group miR187 inhibitorc, the expression of CD276 was upregulated and MMP9 was downregulated(P＜0.01), the proliferation of SGC7901 and the invasion and migration of SGC7901 was inhibited(P＜0.01) in group miR187 inhibitor. Compared with group siRNAnegative control, the expression of MMP9 was upregulated in the group CD276siRNA (P＜0.01). Conclusion: MicroRNA187 was increased in gastric carcinoma and the proliferation and invasion of human gastric cancer cell was promoted by miR187.

收稿日期: 2017-03-16

基金资助:

国家自然科学基金资助项目(81101677，81672913);江苏省自然科学基金资助项目（BK20141288）

通讯作者: 沈慧玲（通讯作者），主任医师，硕士生导师，E-mail:shenhuiling0826@163.com

作者简介: 王馨（1982—），女，主治医师，硕士研究生

引用本文:

王馨1，苏兆亮2，许文林2，沈慧玲1. miR-187在胃癌中的表达及其对胃癌细胞增殖和侵袭的影响[J]. 江苏大学学报:医学版, 2017, 27(5): 391-396. WANG Xin1, SU Zhao-liang2, XU Wen-lin2, SHEN Hui-lin1. Expression of microRNA-187 in gastric carcinoma and its effect on proliferation and invasion of gastric cancer cells. Journal of Jiangsu University(Medicine Edition), 2017, 27(5): 391-396.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2017/V27/I5/391

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