Abstract：Objective: To investigate the role of cordycepin in regulating the inflammatory response of human bronchial epithelial cells and its underlying mechanism. Methods: The human bronchial epithelial cells (16HBE), which grew in good condition, were treated with different concentrations of cordycepin (0, 10, 20, 40, 60 μmol/mL) for 4 hours and then treated with lipopolysaccharide(LPS,1 mg/L) for 12 hours. MTT assay was used for the detection of cell vitality. At the same time, the mRNA expression level of inflammatory cytokines (IL8,TNFα) was evaluated by quantitative realtime PCR. The level of pAKT2 expression was analyzed by Western blotting analysis. Results: Cordycepin could protect bronchial epithelial cells from damage of LPS in a concentrationdependent manner(P<0.05), and attenuated LPSinduced release of proinflammatory cytokines(P<0.05), downregulate the phosphorylation level of AKT2 induced by LPS(P<0.05). Conclusion: Cordycepin shows active inhibitory effect on the inflammatory response of 16HBE cells, possibly through mediating relevant signal pathway of AKT2.
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2017, Vol. 27 
