A pilot study of serum microRNA profiles as a novel biomarker for primary biliary cirrhosis
PAN Teng-li1, 2, SUN Li2, ZHOU Xing-bei2, CHEN Li2, YU Xue-jun2, TAN You-wen2
(1.School of Clinical Medicine, Jiangsu University, Zhenjiang Jiangsu 212001; 2.Department of Liver Diseases, the Third People′s Hospital of Zhenjiang, Zhenjiang Jiangsu 212005, China)
Abstract：Objective: To screen serum microRNA profiles by high throughput sequencing technology method as diagnostic markers for primary biliary cirrhosis (PBC). Methods: First, we employed Illumina GA IIx deep sequencing for the initial screening to indicate the read numbers of microRNAs expression in serum pool of 3 PBC and 3 healthy controls, respectively. Second, qRT-PCR validation study was conducted in more samples. Finally, computer analysis was conducted to predict target genes and biological functions. Results: Twenty-two miRNAs, whose fold change were greater than 2, were filtered out by high throughput sequencing technology. After qRT-PCR validation, the serum expression levels of miR-122, miR-34a and miR-141 were significantly higher in PBC, consistent with the sequencing results. Target genes of prediction results showed that microRNAs played a role in signal transduction, in the nucleus and cytoplasm, through combined with protein. Conclusion: The distinguishing expressed microRNAs had certain reference value on the diagnosis of PBC.
基金资助:
江苏省自然科学基金资助项目(BK2011515)
通讯作者:
谭友文(通讯作者)，主任医师，硕士生导师,E-mail:tyw915@sina.com
作者简介: 潘腾莉(1988—)，女，硕士研究生
引用本文:
潘腾莉1, 2, 孙莉2, 周兴蓓2, 陈丽2, 於学军2, 谭友文2. 血清microRNA表达谱对原发性胆汁性肝硬化的诊断价值[J]. 江苏大学学报:医学版, 2014, 24(3): 230-.
PAN Teng-Li-1, 2 , Sun-Li-2, Zhou-Xing-Bei-2, Chen-Li-2, Yu-Xue-Jun-2, Tan-You-Wen-2. A pilot study of serum microRNA profiles as a novel biomarker for primary biliary cirrhosis. Journal of Jiangsu University(Medicine Edition), 2014, 24(3): 230-.
2014, Vol. 24 
