大豆异黄酮对动脉粥样硬化大鼠炎症分子相关基因表达的影响

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摘要目的: 研究大豆异黄酮(soybean isoflavone , SIF)对动脉粥样硬化(atherosclerosis，As)大鼠血管中促As炎症相关基因表达的影响。方法: 建立As大鼠模型，随机分成正常组、模型组、模型给药组和正常给药组；HE染色观察各组大鼠胸主动脉病理改变；实时PCR和免疫组化检测血管中促As炎症的相关基因表达量。结果：模型组胸主动脉严重萎缩、钙化，出现硬化斑块；模型给药组As病变较模型组明显减轻；正常组和正常给药组胸主动脉无病理改变。模型组胸主动脉中单核细胞趋化蛋白1(monocyte chemotactic protein-1, MCP-1)、CC类趋化因子受体2（C-C chemokine receptor, CCR2）、单核细胞趋化蛋白-1诱导蛋白（MCPIP）和C反应蛋白（CRP）mRNA表达量较正常组明显升高（P<0.01或P<0.05）；模型给药组上述4种基因mRNA表达量均较模型组明显降低（P<0.01或P<0.05）；正常组与正常给药组4种基因mRNA表达量间的差异无统计学意义。免疫组化结果显示，与正常组比较，模型组MCPIP、MCP-1、核因子κB (NF-κB)阳性染色和范围均增多（P<0.01），模型给药组3种蛋白的阳性染色和范围都较模型组明显减少（P<0.05）；正常组和正常给药组3种蛋白表达间的差异无统计学意义。结论：大豆异黄酮能显著降低促As炎症的相关基因表达，明显抑制大鼠As病变。

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关键词 ：动脉粥样硬化, 大豆异黄酮, 单核细胞趋化蛋白-1/CC类趋化因子受体2, 单核细胞趋化蛋白-1诱导蛋白, C反应蛋白, 核因子&kappa, B

Abstract：Objective: To investigate the effects of soy isoflavones(SIF) on the expression of gene for proinflammatory of atherosclerosis(As) in the As rats. Methods: To establish the rats model of As, were randomly divided into normal control, model, model+SIF and normal+SIF group; the pathological changes of thoracic aorta of rats in each group were observed by HE staining; Real time-PCR and immunohistochemical detection of the expression of gene for proinflammatory of As in vessels. Results: The model group showed severe atrophy and calcification of thoracic aorta compared with normal control group, even appear atherosclerotic plaques; compared with model group, model+SIF group showed actions in lessening the atherosclerosis lesion obviously; both normal control group and normal+SIF group without pathological changes of thoracic aorta. Compared with model group, mRNA level of MCP-1/CCR2, MCPIP and CRP were higher than that in normal control group of thoracic aorta（P<0.01 or P<0.05）; mRNA level of the four genes in model+SIF group of thoracic aorta were lower than that in model group; there was not significant difference in mRNA level of the four genes between normal control group and normal+SIF group. Immunohistochemistry results showed that expression of positive staining and range of MCPIP, MCP-1,and NF-κB in model group were higher than in normal control group（P<0.01）, the expression of positive staining and range of the three protein in model+SIF group were lower than in model group（P<0.05）. Between the normal control group and the normal+SIF group, the expression of positive staining and range of the three protein were no significant difference. Conclusion: SIF can obviously decrease the expression of genes related to proinflammatory of As , and inhibit the atherosclerosis formation in rats.

收稿日期: 2014-01-03

基金资助:

遵义医学院2012年招标课题（F-613）

通讯作者: 钱民章（通讯作者），教授，博士生导师， E-mail: qian_mzh@ hotmail.com

作者简介: 罗洁（1986—）,女，硕士研究生；

引用本文:

罗洁1，秦旭1，韦余2，钱民章1. 大豆异黄酮对动脉粥样硬化大鼠炎症分子相关基因表达的影响[J]. 江苏大学学报:医学版, 2014, 24(02): 99-104. LUO Jie1, QIN Xu1, WEI Yu2, QIAN Min-zhang1. Effect of soy isoflavones on the expression of inflammatory molecules gene in atherosclerosis rats. Journal of Jiangsu University(Medicine Edition), 2014, 24(02): 99-104.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2014/V24/I02/99

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