Effects of FoxM1 downregulation by small interfering RNA on chemosensitivity of human colon cancer cell
HE Ya-long1, WANG Xiao-yan1, ZHANG You-li1, FAN Yu2
（1.Department of Gastroenterology, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001； 2.Cancer Institute， the Affiliated People′s Hospital of Jiangsu University， Zhenjiang Jiangsu 212002， China）
Abstract：Objective: To study the effects of Forkhead box protein M1（FoxM1）small interfering RNA（siRNA）on chemosensitivity and mechanism of human colon cancer cell. Methods: Three FoxM1 siRNAs were designed and constructed. The best effect of siRNA downregulation was evaluated by Real time PCR.All cancer cells were divided into different dose of siRNA groups,and RPMI 1640 instead of FoxM1 siRNA as the blank control group (ConA); after transfected for 48 h,all groups cells were treated with irinotecan(Irinotecan, CPT11, 7.5 μmol/L),respectively.The proliferation and chemosensitivity of cancer cells were evaluated by MTT assay,and the phosphorylation of Akt protein was exmined by Western blot.Results: FoxM1 siRNA could downregulate the FoxM1 expression in a dose and time dependent manner. The MTT results showed that the inhibit rates were 18.36%,39.78%,58.18%,78.28% in different groups\[ConA,siRNA 3.125 nmol/L, siRNA 6.25 nmol/L and siRNA 12.5 nmol/L, respectively. Statistical analysis showed that inhibition rates were in a dose dependent(r=0.775，P<0.05）. The phosphorylation of Akt protein was inhibited compared with ConA group. Conclusion: FoxM1 siRNA could sensitize human colon cancer cells to chemotherapy through inducing phosphorylation of Akt protein. ［Key words］colon carcinoma; forkhead box protein M1; chemosensitivity; Akt
何亚龙1, 王晓燕1, 张尤历1 , 范钰2. siRNA下调叉头框M1基因表达对大肠癌细胞化疗敏感性的影响[J]. 江苏大学学报:医学版, 2013, 23(6): 461-.
HE Ya-Long-1, Wang-Xiao-Yan-1, Zhang-You-Li-1 , Fan-Yu-2. Effects of FoxM1 downregulation by small interfering RNA on chemosensitivity of human colon cancer cell. Journal of Jiangsu University(Medicine Edition), 2013, 23(6): 461-.
