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| Influence of pulse ultrasonic on water soluble protein conformation of Tenebrio molitor(L.) larva |
| 1.School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China; 2. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China |
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Abstract The effects of pulse ultrasonic treatment with power of 1 000 W on water soluble protein conformation of Tenebrio molitor (L.) larva were investigated. The results show that the ultraviolet absorption of protein is increased within 25 min ultrasonic treatment. The surface hydrophobicity has remarkable change with maximum value at 10 min，and decreases gradually from 10 to 25 min. The free sulfhydryl (SH) content decreases slightly after pulse ultrasonic treatment for 5 min，and then increases gently. Protein particle size decreases with treatment time from 0 to 15 min, and increases significantly after 20 min treatment. The CD analysis indicates that α-helix content is decreased, while β-structure and random coil content are increased by pulse ultrasonic treatment. SDSPAGE demonstrates that the primary structure of water soluble protein is not changed by pulse ultrasonic treatment.
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2012, Vol. 33 
