非小细胞肺癌患者外周血中生存素和血管内皮细胞生长因子的表达及临床意义

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (746 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To evaluate the clinical significance of mRNA expression of survivin and vascular endothelial growth factor(VEGF) in the peripheral blood from patients with non-small cell lung cancer (NSCLC). Methods: Real-time fluorescence quantitation polymerase chain reaction(RT-PCR) was used to detect mRNA expression of survivin and VEGF in peripheral blood samples from 42 patients with NSCLC and 20 patients with benign lung disease. Results: The mRNA expression of survivin and VEGF in NSCLC patients were significantly higher than those in benign lung disease patients (P=0.006; P=0.001, respectively). The positive rates of mRNA expression of survivin and VEGF in peripheral blood from patients with NSCLC were 59.5 ％(25／42) and 57.1％(24／42), respectively. Combined with the two mRNA expression made the positive rate raise to 76.2％, while the positive rates of mRNA expression of survivin and VEGF in peripheral blood from patients with benign lung disease were 5％(1／20) and 0％, respectively, which were markedly lower than those of patients with NSCLC (both P<0.001). For patients with NSCLC, the tumor TNM stage was significantly correlated with mRNA expression of survivin and VEGF (P<0.001; P=0.008, respectively), whereas there were no correlation between mRNA expression of survivin and VEGF and other pathologic characteristics. Conclusion: The mRNA expression of survivin and VEGF in peripheral blood could be used as the molecular markers for diagnosis of NSCLC; Combined with the two mRNA expression in peripheral blood could raise the detection rate of cancer cell in peripheral blood.

Received: 16 October 2012

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2013/V23/I1/34

［1］ Osaki T, Oyama T, Gu CD, et al. Prognostic impact of micrometastatic tumor cells in the lymph nodes and bone marrow of patients with completely resected stage Ⅰnon-smallcell lung cancer［J］. J Clin Oncol, 2002, 20(13):2930-2936.

［2］ Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene,survivin, expressed in cancer and lymphoma［J］. Nat Med, 1997, 3(8):17-21.

［3］ Tamm I, Wang Y, Sausville E, et al. IAP-family protein survivin inhibits caspase activity and apoptosis induced by Fas(CD90), Bax, caspase, and anticancer drugs［J］. Cancer Res, 1998, 58(23):5315-5320.

［4］ Velculescu VE, Madden SL, Zhang L, et al. Analysis of human transcriptomes［J］. Nat Genet, 1999, 23(4): 387-388.

［5］ Fukuda S, Pelus LM. Survivin, a cancer target with an emerging role in normal adult tissues［J］. Mol Cancer Ther, 2006, 5(5):1087-1098.

［6］ Moussa O, AbolEnein H, Bissada NK, et al. Evaluation of survivin reverse transcriptase-polymerase chain reaction for noninvasive detection of bladder cancer［J］. J Urol, 2006, 175(6):2312-2316.

［7］ Kenney DM, Geschwindt RD, Kary MR, et al. Detection of newly diagnosed bladder cancer, bladder cancer recurrence and bladder cancer in patients with hematuria using quantitative rtPCR of urinary survivin［J］. Tumor Biol, 2007, 28(2):57-62.

［8］ Wu YK, Chen KT, Kuo YB, et al. Quantitative detection of survivin in malignant pleural effusion for the diagnosis and prognosis of lung cancer［J］. Cancer Lett, 2009, 273(2):331-335.

［9］ Lan CC,Wu YK, Lee CH, et al. Increased survivin mRNA in malignant pleural effusion is significantly correlated with survival［J］. Jpn J Clin Oncol, 2010, 40(3):234-240.

［10］ Shen C, Hu LH, Xia L, et al. Quantitative realtime RT-PCR detection for survivin, CK20 and CEA in peripheral blood of colorectal cancer patients［J］. Jpn J Clin Oncol, 2008, 38(11):770-776.

［11］ Cao M, Yie SM, Wu SM, et al. Detection of survivin-expressing circulating cancer cells in the peripheral blood of patients with esophageal squamous cell carcinoma and its clinical significance［J］. Clin Exp Metastasis, 2009, 26(7):751-758.

［12］ Yie SM, Luo B, Ye NY, et al. Detection of Survivin-expressing circulating cancer cells in the peripheral blood of breast cancer patients by a RT-PCR ELISA［J］. Clin Exp Metastasis, 2006, 23(5/6):279-289.

［13］ De Luca A, Pignata S, Casamassimi A, et al. Detection of circulating tumor cells in carcinoma patients by a novel epidermal growth factor receptor reverse transcription-PCR assay［J］. Clin Cancer Res, 2000, 6(4):1439-1444.

［14］ Zhong C, Wei W, Su XK, et al. Serum and tissue vascular endothelial groth factor predicts prognosis in hepatocellular carcinoma patients after partial liver resection［J］. Hepatogastroenterology, 2012, 59(113):93-97.

［15］ Vlems FA, Diepstra JH, Cornelissen IM, et al. Investigations for a multi-marker RT-PCR to improve sensitivity of disseminated tumor cell detection［J］. Anticancer Res, 2003, 23(1A):179-186.

［16］ Chen CC, Chang TW, Chen FM, et al.Combination of multiple mRNA markers (PTTG1, Survivin, UbcH10 and TK1) in the diagnosis of Taiwanese patients with breast cancer by membrane array［J］. Oncology, 2006, 70(4):438-446.

［17］ Pu XY, Wang ZP, Chen YR, et al. The value of combined use of survivin, cytokeratin 20 and mucin 7 mRNA for bladder cancer detection in voided urine［J］. J Cancer Res Clin Oncol, 2008, 134(6):659-665.