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| Effect of lncRNA MALAT1 on Th17/Treg cell balance in asthmatic mice#br# |
| ZHU Haijin1, WANG Zhaoyu1, SHAN Wenqi1, WANG Ying1, CHANG Ming1, WANG Xuefeng2 |
(1. Department of Pediatrics, 2. Department of Central Laboratory, Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China)
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Abstract Objective： To detect the relationship between the long-chain non coding RNA （lncRNA） metastasis associated in lung denocarcinoma transcript 1 (MALAT1) and the balance of Th17/Treg in asthmatic mice. Methods： BALB/c male mice at age of 6-8 weeks were randomly divided into control group and asthma group, with 10 mice in each group. The group of asthmatic mice was established by ovalbumin. HE staining and PAS staining were used to observe the pathological changes of lung tissue. Flow cytometry was used to detect Th17/Treg in spleen cells, and realtime fluorescence quantitative PCR was used to detect the expression level of lncRNA MALAT1 and Rorc/Foxp3 in spleen tissue. Results： Compared with the control group, the airway inflammation of asthmatic mice was significantly changed, with obvious infiltration of inflammatory cells around the bronchus, thickening of the wall, and destruction or irregularity of airway epithelial cells. The proportion of Th17 in the spleen tissue was significantly increased (P<0.01), and the expression of retinoic acid receptor related orphan C (Rorc) was also up-regulated (P<0.05), while the proportion of Treg was significantly decreased (P<0.01), the expression of forkhead/winged helix transcriptional factor P3 (Foxp3) was down-regulated (P<0.05). The relative expression of lncRNA MALAT1 in spleen tissue was significantly increased (P<0.01), positively correlated with the proportion of Th17 cells (r=0.64, P<0.05), and negatively correlated with Treg cells (r=-0.73, P<0.05). Moreover, MALAT1 expression was positively correlated with Rorc expression (r=0.65, P<0.05), and negatively correlated with Foxp3 expression (r=-0.60, P<0.05). Conclusion： MALAT1 is involved in regulating the balance of Th17/Treg in asthmatic mice, which may help to develop therapeutic methods to improve asthma inflammation.
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Received: 08 November 2022
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