聚乳酸羟基乙酸共聚物载脑源性神经生长因子纳米粒的构建及生物学活性评价

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Abstract Objective: To prepare the brain-derived neurotrophic factor (BDNF) poly (lactic-coglycolic acid) copolymer (PLGA) nanoparticles (BDNFPLGANPs) and to investigate its properties and biological activities. Methods: PLGA-NPs were prepared by double emulsion-solvent evaporation method.The formulation process was optimized by orthogonal test according to entrapment efficiency(EE) and drug loading. BDNF-PLGANPs were prepared and characterized under optimized conditions. The cell biocompatibility of BDNF-PLGA-NPs was evaluated by Alarma Blue proliferation assay. Results: The optimal conditions for preparation of BDNF-PLGA-NPs included a volume ratio of primary emulsion to external water phase of 1 ∶15， the mass ratio of dosage to PLGA dosage of 6 ∶100, a concentration of polyvinyl alcohol of 10 g/L and an ultrasonic time of 5 minutes. The BDNF-PLGANPs obtained had a diameter of (205±0.18) nm and an EE of (79.65 ± 2.14)%. The release curve shows that the BDNF-PLGA-NPs have a sustained release effect; BDNFPLGANPs can promote the proliferation of ectomesenchymal stem cells in vitro. Conclusion: BDNF-PLGA-NPs prepared by double solvent volatilization method under optimized conditions have high encapsulation rate, good sustained release effect and biological activity.

Received: 10 April 2020

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	Articles by authors
	LI Yang
	YU Jiangnan
	XU Ximing

Cite this article:

LI Yang,YU Jiangnan,XU Ximing.

Preparation and bioactivity evaluation of brain-derived neurotrophic factor-loaded poly (lactic-co-glycolic acid) nanoparticles

[J]. Journal of Jiangsu University(Medicine Edition), 2021, 31(01): 28-32，37.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2021/V31/I01/28

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