白藜芦醇通过诱导GMDSCs凋亡和MMDSCs分化延缓Lewis荷瘤小鼠肿瘤生长

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Abstract Objective: To investigate the influence of resveratrol on myeloidderived suppressor cells (MDSCs) and its role in antitumor immunity. Methods: The Lewis lung carcinoma tumorbearing mice models were established and orally treated with resveratrol(resveratrol group, n=6) and PBS(control group, n=6) respectively, the volumes and weights of tumors were measured; the proportions of different subsets of MDSCs infiltrated in spleens and tumor tissues of tumorbearing mice were examined by flow cytometry(FCM). GMDSCs and MMDSCs were isolated from splenocytes of tumorbearing mice by magneticactivated cell sorting (MACS). After stimulated with resveratrol for 12 h, FCM was used to examine the apoptosis of GMDSCs and the differentiation of MMDSCs. Results: Compared with the control group, the tumor volumes and weights of tumorbearing mice in resveratrol group were significantly decreased(P<0.01,P<0.05); the results of FCM indicated that the percentages of total MDSCs and MMDSCs in spleens of tumorbearing mice showed no significant difference, while the percentages of GMDSCs were reduced; and in tumor tissues, the frequencies of both total MDSCs and GMDSCs were decreased, but the frequencies of MMDSCs showed no marked difference. The isolated GMDSCs and MMDSCs were separately treated with resveratrol for 12h, the early and late apoptotic GMDSCs were higher than those in control group and the expression levels of CD11c and F4/80 on MMDSCs were both significantly upregulated. Conclusion: Resveratrol may inhibit tumor growth by promoting the apoptosis of GMDSCs and the differentiation of M-MDSCs.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2019/V29/I01/26

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