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Extraction and antioxidant activities in vitro of polysaccharides from the seeds of Momordica charantia L |
LI Hui-hua, YU Jiang-nan, XU Xi-ming |
(School of Pharmacy, Jiangsu University, Zhenjiang Jiangsu 212013, China) |
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Abstract Objective: To study the extraction technology and antioxidant activities in vitro of polysaccharides from the seeds of Momordica charantia L(bitter melon seeds). Methods: The content of polysaccharides from bitter melon seeds was determined by phenolsulfuric acid method. On the basis of single factors experiments, the four factors including extraction temperature, extraction time, solidliquid ratio and extraction times on the extraction rate of polysaccharides from bitter melon seeds were studied by orthogonal experimental design.DPPH radical, hydroxyl radical,superoxide anion radical and total reduction abilities were used to reflect the antioxidant abilities of polysaccharides.Results: Temperature was an important factor affecting the extraction rate of polysaccharides from bitter melon seeds. The optimal extraction technology conditions were that extraction temperature was 90 ℃, solidliquid ratio was 1 ∶30, the extraction time was 2.5 h, extraction times were 3 times. The extraction rate of polysaccharides were 5.61%. Compared with vitamin C polysaccharides from the seeds of bitter melon had stronger scavenging ability to DPPH radical and hydroxyl radical, but weaker ability to scavenging superoxide anion radical and total reduction ability. Conclusion: This extraction technology is suitable for extraction of polysaccharides from seeds of Momordica charantia L. The polysaccharides have good antioxidant activity at a high concentration.
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Received: 17 May 2018
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