浆细胞瘤转化迁移基因1在结直肠癌患者血浆中的表达与临床意义

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Abstract Objective: To investigate the expression level of long noncoding RNA（lncRNA） plasmacytoma variant translocation 1 gene(PVT1) in plasma of patients with colorectal cancer and its clinical significance. Methods: The plasma samples were collected from 90 patients with colorectal cancer, 30 patients with colorectal adenoma, 42 healthy controls and 29 matched colorectal cancer patients for 2-3 weeks after operation. The expression of lncRNA PVT1 was detected by quantitative real time PCR(qRTPCR)in all samples and 29 patients with colorectal cancer were compared before and after operation. The correlation between plasma PVT1 expression and clinicopathological parameters of colorectal cancer was further analyzed, and receiver operating characteristic (ROC) curve was used to evaluate the diagnostic efficiency of plasma PVT1 for colorectal cancer. Results: The expression levels of plasma PVT1 in the colorectal adenoma group, the colorectal cancer group and the relative early TNM Ⅰ/Ⅱ colorectal cancer group were all significantly higher than those in the health group (all P<0.05) and decreased significantly after operation (P<0.01). And they were not correlated with the age, sex and tumor location, size, differentiation degree, TNM stage (all P>0.05). All diagnostic efficacy of the plasma PVT1 in the diagnosis of colorectal cancer were higher than conventional tumor marker carcinoembryonic antigen(CEA). The diagnostic value of combined detection of PVT1 and CEA for both colorectal cancer and TNM Ⅰ/Ⅱ colorectal cancer were higher than that of PVT1 and CEA alone. Conclusion: The upregulated plasma lncRNA PVT1 expression in patients with colorectal cancer and precancerous lesions could be served as a potential circulating marker for the clinical diagnosis and postoperative monitoring of colorectal cancer.

Received: 22 January 2018

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2018/V28/I04/307

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