PIK3CD-AS1对肾癌细胞增殖和凋亡的影响

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Abstract Objective: To investigate the expression of lncRNA PIK3CDAS1 in renal cancer cells and its effect on the proliferation and apoptosis of renal cancer cells,and the genes related to the function of PIK3CDAS1 were explored. Methods: The expression of PIK3CDAS1 in normal kidney cells(HK2) and renal cancer cells (769P, 786O, A498) was detected by realtime fluorescence quantitative PCR(qRTPCR). The PIK3CDAS1 overexpression vector was transfected into renal cancer cells(769p) to construct a PIK3CDAS1 stable overexpression cell line. The effect of PIK3CDAS1 on the proliferation and apoptosis of renal cancer cells was detected by CCK8 assay and flow cytometry. The apoptosisrelated gene expression profile chip was used to screen genes that may be related to PIK3CDAS1 function. Results: The expression of PIK3CDAS1 was significantly decreased in kidney cancer cells (769P, 786O, A498) compared with normal renal cell HK2 (P<0.01 or P<0.05). The PIK3CDAS1 overexpression cell line was successfully constructed and verified by qRTPCR. The expression of PIK3CDAS1 in the experimental group was significantly higher than that of the negative control (P<0.05). The proliferation and apoptosis experiments showed that the proliferation ability of the cells in the experimental group was significantly decreased (P<0.01), and the apoptosis was significantly increased (P<0.01). The results of gene expression microarray screening showed that the genes interacting with PIK3CDAS1 and promoting apoptosis are DFFA, BCL2L1, and CASP9. Conclusion: PIK3CDAS1 may be a tumor suppressor gene and is lowly expressed in renal cancer cells. PIK3CDAS1 can inhibit the proliferation of renal cancer cells and promote apoptosis, and its proapoptotic function may be related to CASP9, DFFA, BCL2L1 genes.

Received: 19 March 2018

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2018/V28/I04/286

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