血管紧张素Ⅱ对结肠癌CT26细胞株增殖及侵袭的影响

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Abstract Objective: To investigate the effect of angiotensin Ⅱ ( Ang Ⅱ) and angiotensin Ⅱ type 1 receptor (AT1R) blocker losartan on the proliferation and invasion of CT26 cells. Methods: The expression of AT1R was detected by immunofluorescence assay. The proliferation in cells treated with various concentration of Ang Ⅱ and losartan was examined by CCK8 assay. The CT26 cells were divided into three groups, control group(routine culture), Ang Ⅱ group (1×10-7moL/L) and losartan+Ang Ⅱ group (first treated with 1×10-6moL/L losartan for 2 h, then treated with 1×10-7moL/L Ang Ⅱ). The capacity of cell invasion was detected by invasion assay. The secretion of TNF α was detected by ELISA assay. The expression of AT1R and TNFα was determined by Western blotting. Results: The immunofluorescence result showed that AT1R was expressed in CT26 cell. Compared with the control group, Ang Ⅱgroup promoted the proliferation and invasion of CT26 cells, upregulated the expression of AT1R and TNFα(all P<0.05). Compared with Ang Ⅱ group, losartan +Ang Ⅱgroup significantly inhibited the proliferation and invasion of CT26 cells, downregulated the expression of AT1R and TNFα (all P<0.05). Conclusion: Ang Ⅱ could promote proliferation and invasion of CT26 cells, while losartan could antagonize the effect induced by Ang Ⅱ.

Received: 11 April 2018

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2018/V28/I04/277

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