不同热剂量等级的微波消融治疗对结肠癌小鼠T细胞的影响

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1183 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To investigate the effect of microwave ablation with different thermal dosages on T cells in mice with colon cancer. Methods: MC38 colon cancer models of tumorbearing mice were established and thereafter, randomized to the the control group and microwave ablation group, which were divided into low（5 W,60 s）, moderate（10 W,60 s）, and high（15 W,60 s） dose groups. On the 9th day after colon tumor cells implantation, the mice underwent the microwave and the tumor growth were observed. On 14th day of microwave ablation, CD3+T, CD4+T, and CD8+ T cells in the spleens of mice were collected and detected by flow cytometry. Results: The tumor volumes of mice in microwave ablation groups were decreased compared with those in the control group(P<0.05). The percentages of CD3+T, CD4+T cells in spleens of mice in microwave ablation groups were significantly increased versus those in control group(P<0.05 or P<0.01), especially in the high dose group(P＜0.05). Further, the percentages of CD4+/CD8+ T cells in spleens of mice in high dose group was elevated versus those in moderate dose group and low dose group(P<0.05), as well as the control group（P＜0.01）. The percentages of CD8+T cells were insignificant in each group（P>0.05）. Conclusion: Microwave ablation can activate the immune functionality and inhibit the tumor growth in mice with colon cancer, and higher dose, more effective.

Received: 31 October 2017

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2018/V28/I02/117

［1］孙敬国，蒋晓忠.Twist蛋白表达与结肠癌发生发展的关系研究\[J\].中国全科医学，2011，4 (14)：1311-1314.
［2］郑灵巧，马彦明.大肠癌发病率不断攀升\[J\].老年健康，2011(5)：4.
［3］Simoglou C，Gymnopoulou I，Babalis D，et al. Surgery of colon cancer in a district hospital \[J\].Hell J of Surg，2012，84 (1) ：71-75.
［4］Whiteford MH，Whiteford HM，Yee LF，et al．Usefulness of FDGPET scan in the assessment of suspected metastatic or recurrent adenocarcinoma of the colon and rectum［J］．Dis Colon Rectum，2000，43(6):759-767．
［5］Yue WW, Wang SR,Lu F,et al. Radiofrequency ablation vs. microwave ablation for patients with benign thyroid nodules: a propensity score matching study\[J\].Endocrine,2017,55(2):485-495.
［6］Korkusuz H, Happel C, Koch DA, et al. Combination of ultrasoundguided percutaneous microwave ablation and radioiodine therapy in benign thyroid disease: a 3month followup study\[J\].Rofo, 2016,188(1):60-68.
［7］CornmanHomonoff J,Miller ZA,Smirniotopoulos J,et al.Preoperative percutaneous microwave ablation of long bone metastases using a parallel medullary approach for reduction of operative blood loss\[J\].J Vasc Interv Radiol,2017,28(7):1069-1071.
［8］Huang H,Hao S,Li F,et al.CD4+Th1 cell promote CD8+T cell survival, memory response, tumor localization and therapy by targeted delivery of interleukin 2 via acquired pMHCI complexes［J］.Immunology,2007,120(2): 148-159.
［9］Oizumi S, Strbo N, Pahwa S, et al. Molecular and cellular requirement for enhanced antigen crosspresentation to CD8 cytotoxic T lymphocytes［J］.J Immunol,2007,179(4): 2310-2317.
［10］LardiereDeguelte S, De Mestier L,Amroun KL,et al.Laparoscopic thermal ablation of splenic metastases initial experience and present aspects［J］.J Visc Surg,2013, 50(5): 355-358.
［11］Liu QD,Song Y,Zhou NX,et al. Radiofrequency ablation of splenic tumors: a case series［J］.J Gastrointestin Liver Dis,2013,22(1): 105-108.
［12］Zerbini A, Pilli M, Penna A, et al. Radiofrequency thermal ablation of hepatocellular carcinoma liver nodules can activate and enhance tumorspecific Tcell responses［J］.Cancer Res, 2006, 66(2):1139-1146.
［13］Pashenkov M,Goss G,Wagner C,et al.Phase Ⅱ trial of a tolllike receptor 9activating oligonucleotide in patients with metastatic melanoma［J］.J Clin Oncol,2006, 24（36）: 5716-5724.
［14］Lonsdorf AS,Kuekrek H,Stern BV,et al.Intratumor CpGoligodeoxynucleotide injection induces protective antitumor T cell immunity［J］.J Immunol, 2003, 171（8）: 3941-3946.