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| Expression of circulating microRNA-195 and its clinical significance in patients with cervical cancer and cervical intraepithelial neoplasia |
| ZHAO Jing |
| (Department of Obstetrics and Gynecology, the Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212132, China) |
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Abstract Objective: To evaluate the expression of circulating microRNA-195(miR-195) and its clinical significance in patients with cervical cancer(CC) and cervical intraepithelial neoplasia(CIN). Methods: Real-time quantitative PCR was performed to examine serum miR-195 expression levels in 30 healthy controls, 51 CIN patients and 51 CC patients. Receiver operating characteristic curve(ROC) were conducted to assess the value of the level of miR-195 expression in distinguishing CIN and CC patients from healthy controls. The impact of miR-195 expression on prognosis in CC patients was assessed by Kaplan-Meier method. Results: There were significant differences in the levels of miR-195 between CC patients, CIN patients and the healthy controls( P<0.01); and the level of CC patients were significantly lower than those of the other two groups(P<0.01 or P<0.05). ROC analyses revealed that the level of miR-195 expression was a potential biomarker in discriminating CC patients or CIN patients from controls. Moreover, Kaplan-Meier analysis demonstrated that miR195 low-expressed patients had shorter overall survival time than miR-195 high-expressed patients (P=0.016). Conclusion: The levels of serum miR-195 expression in peripheral blood of patients with cervical cancer and CIN could be used as a molecular marker for the diagnosis of cervical cancer and CIN. ［Key words］microRNA195; diagnosis; cervical cancer; cervical intraepithelial neoplasia
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Received: 21 April 2017
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2017, Vol. 27 
