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| Change and significance of CD8+CD25+Foxp3+Treg cells in peripheral blood of patients with asthma |
| YU Jian-xiu 1, QIAN Lei 1, CHENG Guo-ping 2, ZHU Jing-he 2 |
| (1. Department of Laboratory Medicine; 2. Department of Respiratory Medicine, Binhai People′s Hospital, Yancheng Jiangsu 224500, China) |
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Abstract Objective: To detect the expression of CD8+CD25+Foxp3+ regulator T cells (CD8+Treg) in asthma patients, and to investigate the role of CD8+Treg in the pathogenesis of asthma patients. Methods: The study included 50 patients with asthma, 25 patients with mild-to-moderate asthma(MA), 25 patients with severe asthma(SA) and 25 age-matched healthy controls. The percentage of CD8+Treg cells was detected by flow cytometry. The Foxp3 mRNA of peripheral blood mononuclear cell was determined by RT-PCR. The cytokines of IL-33, IL-1β and transforming growth factor-β1(TGF-β1) were detected using Luminex200. Forced expiratory volume in 1 second(FEV1) and peak expiratory flow(PEF) were detected by Pulmonary Function Testing Systems. Results: Compared with healthy controls\[6.48(5.21-6.92)%], MA patients [4.13(2.05-5.41)%] and SA patients\[2.32(1.24-3.63)%] had lower percentages of CD8+Treg cells(P<0.05). Compared to healthy controls, higher levels of IL-33 were found in SA and MA patients and much higher in SA patients (P<0.05). Compared to healthy controls and MA group, SA patients showed higher levels of TGF-β1 and IL-1β. The percentage of CD8+Treg cells showed a positive correlation with FVE1(r=0.411, P=0.003) and PEF (r=0.441, P=0.001) in asthma patients. The ROC curve analysis showed the CD8+Treg had high specificity and sensitivity in the diagnosis of asthma. Conclusion: The decreased CD8+Tregs might play a role in the pathogenesis of asthma. ［Key words］asthma; CD8+CD25+Foxp3+Treg; FEV1; peak expiratory flow
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Received: 12 May 2017
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| [1] |
. [J]. Journal of Jiangsu University(Medicine Edition), 2019, 29(05): 447-450. |
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2017, Vol. 27 
