胃癌间质干细胞敲减YAP抑制胃癌的发展

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Abstract Objective: To investigate the effects of YAP knockdown in gastric cancerderived mesenchymal stem cell（GCMSC） on proliferation, migration and angiogenesis of gastric cancer. Methods: For YAP knockdown, the lentiviralYAP were infected into GCMSC. qRTPCR and Western blotting were used to test the efficacy of YAP knockdown. The mRNA expression of VEGF, PDGF and IL8 were tested by qRTPCR. By collecting the conditioned medium(CM) of GCMSC YAP shRNA group and control group, the change of angiogenesis was detected by using the endothelial tube formation assay. Colony formation assay and cell migration assay were used to test the proliferation and migratory ability of SGC7901 treated with the CM of YAP knockdown GCMSC. Results: Compared with the control group, the protein and gene expression levels of YAP decreased significantly (P<0.01). The decreased expression of vascular endothelial growth factor (VEGF), platelet derived growth factor(PDGF) and IL8 were also observed (P<0.01). The angiogenesis ability of YAP knockdown GCMSC decreased significantly. YAP knockdown in GCMSC could inhibit the proliferation and migration of SGC7901. Conclusion: YAP knockdown in GCMSC inhibited angiogenesis and reversed the promotion of GCMSC on the proliferation and migratory of SGC7901. It could be implicated that YAP may be involved in the angiogenesis, proliferation and migration of gastric cancer cells.

Received: 11 April 2017

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2017/V27/I5/374

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