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| Effect of doxycycline on proliferation of human multiple myeloma cells |
| YAN Ling-ling 1, FEI Xiao-ming 1, 2, TANG Yu 3, YE Wei 1, LU Ping1 , ZHU Yan 1 |
| （1. Department of Hematology, 2. Department of Blood Transfusion, 3. Department of Rheumatism, Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China) |
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Abstract Objective: To investigate anti-myeloma effect of doxycycline (DOX) and its possible mechanism. Methods: MTT assay was employed to evaluate antimyeloma effect of DOX on human myeloma cell line H929 and U266, and then protein expression level of PARP, p-Stat1 and p-Erk1/2 were measured by Western blotting analysis when pre- or post-exposure to DOX. Results: Myeloma cell lines U266 and H929 proliferation was inhibited by DOX treatment in a time- and dose-dependent manner. DOX suppressed the expression of p-Erk1/2 in both multiple myeloma cell lines in dose-dependent manner. DOX was only showed to up-regulate p-Stat1 in H929. Compared to the control group, there was no significant difference in the expression level of p-Stat1 in U266 cell and PARP in both H929 and U266 cells. Conclusion: DOX exhibited antimyeloma effect, which might be contributed to Stat and Erk pathways regulation.
［Key words］multiple myeloma; doxycycline; proliferation; p-Stat1;p-Erk1/2
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Received: 29 March 2017
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2017, Vol. 27 
