重组新城疫病毒rL-29抑制裸鼠A549细胞瘤体生长

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Abstract Objective: To explore the effect of recombinant newcastle disease virus expressing IL29(rL29) on growth of tumorbearing mouse model and the underlying mechanisms. Methods: A tumor model of human A549 into nude mice was constructed and divided randomly into three groups, including rL29 group, newcastle disease virus(NDV) group and PBS group(n=10 each). The tumor of each group were injected with rL29, NDV, PBS for 4 weeks respectively, and twice per week. Immunohistochemistry were performed to detect the expression of IL29 in tumor. Tumour volume was measured, and draw growth curves. Western blotting was performed to detect the expression of endoplasmic reticulum stress, autophagy and apoptosis associated proteins. Results: In rL29 group, IL29 protein stably expressed. Compared with NDV and PBS group,the growth of tumor in rL29 group were inhibited stronger. The expression of endoplasmic reticulum stress associated proteins such as grp78, peIF2α, CHOP and autophagy associated proteins such as Beclin1, LC3, apoptosis associated protein such as cleaved caspase3 significantly upregulated. Conclusion: rL29 effectively inhibited the growth of tumor, which may be attributed to activation of endoplasmic reticulum stress, autophagy and apoptosis.

Received: 01 March 2017

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2017/V27/I02/133

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