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| Expression of TNF-α/NF-κB signaling pathway in the bone of systemic lupus erythematosus mice (MRL/lpr) |
| CUI Ting1, SONG Dong-ming1, QIU Ying-ying1, RUI Jin-bing1, WU Ling1, FEI Xiao-ming2, LI Jing1, TANG Yu1 |
| (1. Department of Rheumatology and Immunology, 2. Department of Hematology, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China) |
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Abstract Objective: To investigate the tumor necrosis factor-α(TNF-α)/nuclear factor-κB p50 (NF-κB p50)signaling pathway in bone of systemic lupus erythematosus mice (MRL/lpr). Methods: Six C3He/HeJ mice (control group) and six MRL/lpr mice (lupus group) were feed, then mouse femur tissue sections were prepared and bone circumstances were observed by hematoxylin-eosin (HE) staining. The proteins of TNF-α and NF-κB p50 were detected by immunohistochemical staining. Bone marrow mesenchymal stem cells (BMMSCs) were isolated by density gradient centrifugation adherence screening method and cultured, then the expressions of TNF-α, NF-κB p50 were detected by immunocytochemistry staining. Results: Trabecular bone of MRL/lpr lupus mice showed osteoporosis and the percentage of the cortex to the volume of bone of MRL/lpr mice were significantly lower compared to control group (P<0.05). The protein expression levels of TNF-α and NF-κB p50 on the femur of MRL/lpr mice were higher than the C3He/HeJ mice (P<0.05 or P<0.01). Immunocytochemistry results displayed that on the BMMSCs of the MRL/lpr mice, the protein expression levels of TNF-α and NF-κB p50 were also higher than the C3He/HeJ mice (P<0.05 or P<0.01). Conclusion: TNF-α/NF-κB signaling pathway on the bone of lupus mice has been abnormally activated.
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Received: 23 July 2014
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2014, Vol. 24 
