Abstract：Objective： To investigate the susceptibility of insulin resistance-Buffalo rat liver (IR-BRL) cells to acrylonitrile toxicity and the attenuation effect of diet-based chemical phenethyl isothiocyanate (PEITC). Methods： Treat BRL cells with 50 nmol/L insulin for 24 h to construct the IRBRL cell model with considering glucose consumption, insulin receptor substrate-2 (IRS-2) protein and intracellular phosphatidylinositol-3 kinase (PI3K) protein expression as evaluation indicators. IR-BRL cells treated with 2.5-5.0 mmol/L acrylonitrile for 12 h, and the cell viability and cytotoxicity were assessed by MTT reduction and lactate dehydrogenase (LDH) leakage respectively to evaluate the susceptibility characteristics of IR-BRL cells to acrylonitrile and then the above acrylonitrile-exposed IR-BRL cells was pretreated with 1.25-5.0 μmol/L PEITC for 1 hour and repeat the above examination to evaluate its attenuation effect. Results： The glucose consumption and the expression of IRS2 and PI3K proteins of BRL cells treated with insulin has shown significantly reduced, indicating the successful establishment of insulin resistance model of BRL cells. Compared with normal BRL cells, the IR-BRL cells treated with 2.5 and 4.0 mmol/L acrylonitrile showed significantly lower cell viability and higher LDH leakage rate, indicating that IR-BRL cells were more sensitive to acrylonitrile. Compared with normal infected hepatocytes, the pretreatment of 1.25-5.0 μmol/L PEITC could significantly reverse the above indicators. Conclusion： IRBRL cells are more sensitive to acrylonitrile, and PEITC may decrease its susceptibility.
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收稿日期: 2022-02-11
基金资助:国家自然科学基金资助项目（81302419）
通讯作者:
李芳（通讯作者），副教授，硕士生导师，E-mail： lfsjy@ujs.edu.cn
作者简介: 朱薛羽（1995—），女，硕士研究生
引用本文:
朱薛羽, 周结, 董颖, 陆荣柱, 李芳. 丙烯腈对胰岛素抵抗大鼠肝细胞的毒性影响及苯乙基异硫氰酸酯的保护作用[J]. 江苏大学学报:医学版, 2023, 33(02): 135-140.
ZHU Xueyu1, ZHOU Jie1, DONG Ying2, LU Rongzhu1, LI Fang1. Acrylonitrile-induced cytotoxicity in insulin resistance-Buffalo rat liver cells: protective effect of phenylethyl isothiocyanate. Journal of Jiangsu University(Medicine Edition), 2023, 33(02): 135-140.
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