寻常型银屑病患者外周血线粒体DNA检测及其临床意义

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摘要目的：分析寻常型银屑病患者外周血线粒体DNA（mtDNA）水平，并探讨其在疾病中的作用机制。方法: 收集28例寻常型银屑病患者和28例健康对照者外周血，实时荧光定量PCR检测血浆mtDNA表达。分离外周血单个核细胞，实时定量PCR检测环状GMP-AMP合酶（cyclic GMPAMP synthase,cGAS）和干扰素基因刺激物（stimulator of interferon gene,STING）mRNA表达水平。外周血单个核细胞与mtDNA共培养，ELISA检测上清液白细胞介素（interleukin,IL）-23、IL-17、干扰素-γ（interferon-γ,IFN-γ）浓度。结果: 与健康对照组相比，银屑病组外周血mtDNA相对表达显著升高\[(2.34±0.81) vs (1.18±0.25)，P<0.001］，且与银屑病皮损面积和严重程度指数（PASI）评分呈正相关（r=0.693,P<0.001）。与健康对照组相比，银屑病组外周血单个核细胞cGAS和STING mRNA相对表达均显著上升（P均<0.001）。与mtDNA共培养后，单个核细胞IL-23、IL-17、IFN-γ分泌显著增加（P均<0.001），且与PASI评分均呈正相关（r分别为0.647，0.585，0.492，P均<0.01）。结论: 银屑病患者外周血mtDNA异常升高，可能通过活化免疫细胞cGAS/STING信号通路，参与银屑病疾病过程。

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	曹阳
	申宇鸿
	宋世珅
	钱青

关键词 ：银屑病, 线粒体DNA, 环状GMP-AMP合酶, 干扰素基因刺激物

Abstract：Objective： To investigate the level of mitochondrial DNA (mtDNA) in peripheral blood of patients with psoriasis vulgaris and its mechanism in the disease. Methods： The peripheral blood of 28 patients with psoriasis vulgaris and 28 healthy controls were collected, and the plasma mtDNA level was detected by quantitative real-time PCR (qRT-PCR). The peripheral blood mononuclear cells (PBMCs) were isolated, and the mRNA expression levels of cyclic GMPAMP synthase (cGAS) and stimulator of interferon gene (STING) were detected by qRT-PCR. PBMCs were co-cultured with mtDNA, and the concentrations of interleukin (IL)-23, IL-17 and interferon (IFN)-γ in the supernatant were detected by enzyme-linked immunosorbent assay. Results： Compared with the healthy group, the mtDNA expression in the peripheral blood of the psoriasis group was significantly increased ［(2.34±0.81) vs (1.18±0.25), P<0.001］, and was positively correlated with the psoriasis area and severity index (PASI) score (r=0.693, P<0.001). Compared with the healthy group, the levels of cGAS and STING mRNA in PBMCs of the psoriasis group were significantly increased (both P<0.001). The concentrations of IL-23, IL-17 and IFN-γ in the supernatant of PBMCs cocultured with mtDNA were significantly higher than those in the blank control group (all P<0.001) and positively correlated with PASI score (r=0.647, 0.585, 0.492, all P<0.01). Conclusion： The mtDNA in peripheral blood of patients with psoriasis is abnormally elevated and may participate in the process of psoriasis by activating cGAS/STING signaling pathway in immune cells.

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收稿日期: 2022-09-19

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通讯作者: 钱青（通讯作者），主任医师，E-mail: qianqinghpl@163.com

作者简介: 曹阳（1990—），女，主管技师

引用本文:

曹阳，申宇鸿，宋世珅，钱青. 寻常型银屑病患者外周血线粒体DNA检测及其临床意义[J]. 江苏大学学报:医学版, 2023, 33(02): 126-129,134. CAO Yang1, SHEN Yuhong2, SONG Shishen2, QIAN Qing2. Significance and detection of the mitochondrial DNA in peripheral blood from patients with psoriasis vulgaris. Journal of Jiangsu University(Medicine Edition), 2023, 33(02): 126-129,134.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2023/V33/I02/126

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