Abstract：Objective: To investigate the effect of gossypol acetate （GAA） on ferroptosis in human glioma cells. Methods: Human brain glioma U87MG, LN229 and U251MG cells in logarithmic growth phase were selected and divided into two groups, treated with 0 or 10 μmol/L GAA, respectively, CCK-8 and clony formation assays were used to detect cell proliferation abilities. QRT-PCR and Western blotting were used to detect the expression of glutathione peroxidase 4（GPX4） in mRNA and protein levels, respectively. Also, the contents of reduced glutathione （GSH） and malondialdehyde （MDA） were detected by chemiluminescence. In addition, three kinds of human glioma cells were co-cultured with 0 or 10 μmol/L GAA combined with 0, 1 μmol/L Ferrostatin-1, respectively, and the cell proliferation rate was detected by CCK-8 method. Results: Compared with 0 μmol/L GAA group, the relative proliferation rate and clonal formation ability of U87MG, LN229 and U251MG cells in GAA 10 μmol/L group were significantly decreased （all P<0.05）, the expression levels of GPX4 mRNA and protein in LN229 and U251MG cells decreased significantly （all P<0.05）, the content of GSH decreased significantly and the content of lipid peroxidation product, malondialdehyde increased remarkably（P<0.05）, while the above indexes in U87MG cells did not change greatly （P>0.05）. Compared with 10 μmol/L GAA+0 μmol/L Ferrostain-1 group, the relative proliferation rate of three brain glioma cells in 10 μmol/L GAA+1 μmol/L Ferrostatin-1 group was significantly increased（all P<0.05）. Conclusion: Gossypol acetate promotes ferroptosis in glioma cells, and LN229 and U251MG cells are more sensitive than U87MG cells.
［Key words］glioma; gossypol acetate; ferroptosis;Ferrostatin-1; glutathione peroxidase 4（GPX4）
收稿日期: 2022-01-13
基金资助:江苏省研究生科研创新计划立项（KYCX21_3400）
通讯作者:
许潇（通讯作者）,讲师,硕士生导师
作者简介: 杨洋（2001—）,女,本科生；
引用本文:
杨洋, 杨梦婷, 许潇. 醋酸棉酚通过诱导铁死亡抑制脑胶质瘤细胞生长[J]. 江苏大学学报:医学版, 2022, 32(03): 246-250.
YANG Yang, YANG Mengting, XU Xiao. Inhibitory effect of gossypol acetate on the growth of glioma cells via induction of ferroptosis. Journal of Jiangsu University(Medicine Edition), 2022, 32(03): 246-250.
