Abstract：［Abstract］Objective： To explore the change of the expression of glucocorticoid receptor(GR) in the hippocampus of rat model of depression by chronic unpredictable mild stress(CUMS). Methods：A rat model of depression was established with CUMS method.The rat hippocampus tissues at week 2 and 4 and 6 after CUMS were extracted and the normal hippocampus tissues used as control were collected.The expressions of GR in the hippocampus in each group were observed by the immunofluorescent staining,and RT-PCR method was applied to measure the expression of GR mRNA in hippocampus.Using MRI and HE staining method was used to analyze the morphological and structural changes of hippocampus of each group. Results：GR was expressed in abundance in control group and fluorescence intensity was high.The expressive cell arranged closely and regularly. Compared with the control group,as the stress going on, the fluorescence intensity of GR expression in depressive hippocampus gradually weakened and the number of GR expression cell gradually fell, the morphological of hippocampus cell had become disorder. GR-positive cell number was significant lower than control group(P<0.01).The expression of GR mRNA in the depressive group was significantly reduced compared to the control group(P<0.01).The morphological and structural changes of hippocampus had the significant change in the depressive group. The hippocampus cells of rats at week 2 and 4 and 6 after stress were arranged disorderly and the count decreased,the morphological and structural had become irregular. Conclusion：The decrease of GR in the hippocampus of rat model of depression by CUMS may be one of the underlying mechanisms to explain the occurrence of hippocampal neurons damage.
孙美芳，李月峰，王冬青，殷瑞根，赵天，陈芸. 慢性不可预见性应激模型大鼠海马形态及其
糖皮质激素受体表达的动态观察[J]. 江苏大学学报:医学版, 2016, 26(05): 369-374.
SUN Mei-fang，LI Yue-feng，WANG Dong-qing，YIN Rui-gen, ZHAO Tian，CHEN Yun. Dynamic change of the expression of glucocorticoid receptor in the hippocampus of rat model of depression by chronic unpredictable mild stress. Journal of Jiangsu University(Medicine Edition), 2016, 26(05): 369-374.
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2016, Vol. 26 
