Abstract：［Abstract］Objective： To develop and identify adenovirus AdEGFPhTSHR289. Methods：The hTSHR289 gene was obtained by double digestion pBluescript Ⅱ SK(+)hTSHR289 and pAdTrack CMV with NotⅠ/XhoⅠ, and inserted into predigested plasmid pAdTrackCMV. The linearized plasmid pAdTrackhTSHR289 was transfected into E.coli BJ5183 containing pAdEasy1. The recombinant plasmid pAdEasyGFPhTSHR289 was transfected into 293A cells for getting recombinant adenovirus AdEGFPhTSHR289. The virus titer was detected by TCID50 method. Results： hTSHR289 was successfully inserted into adenovirus vector, and obtained the recombinant adenovirus vector. The adenovirus carrying hTSHR289 gene were acquired by packaging, the titer of adenovirus was about 3.5×109 pfu/ml. Conclusion： The recombinant adenovirus carrying hTSHR289 were sucessfully developed.
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2016, Vol. 26 
