Abstract To investigate inflow fields in Micro-channel at Reynolds number lower than 1,such as inflow in vascular plant,with micro-fluidic particle image velocimetry(Micro-PIV) technique and numerical simulation that simulating wall roughness elements by setting up porous medium thickness and momentum source of porous medium model in software Fluent,inflow fields in straight micro-channels with cross section of 400 μm×400 μm under the conditions of the Reynolds number of 0.15,0.25 and 0.35 were obtained respectively.The velocity distributions were compared with analytical solution adopted by solving governing equations directly.The result shows that effect from wall roughness on the velocity distributions is small but not ignorable in micro-channel,so the analytical solution cannot meet the experimental results.The profile has the same tendency,but the value is lower in the mean flow area,which is distant from horizontal axis less than 0.04 mm,and higher in other region near the wall.Velocity distributions derived by the numerical simulation meet the experimental results well,so this numerical simulation can be successfully used in handling inflow problems at low Reynolds number in square section straight micro-channel.
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